Ihc staining is accomplished using antibodies that recognize a target protein and antibody antigen complexes that are visualized via chromogenic radioactive or fluorescent substrates these can be introduced to the reaction as direct primary conjugates or via a secondary antibody there are a variety of techniques for sample preparation and . Indirect fluorescent antibody techniques indirect fluorescent antibody ifa tests figure 2 are used to look for antibodies in patient serum for example an ifa test for the diagnosis of syphilis uses t pallidum cells isolated from a lab animal the bacteria cannot be grown on lab media and a smear prepared on a glass slide. It clearly presents detailed easy to follow step by step methods for the widely used techniques that exploit the unique properties of antibodies and will help researchers use antibodies to their maximum advantage. Overview of antibody production methods and levels of antibody purification introduction to antibody production and purification introduction to antibody production and purification the ability of animal immune systems to produce antibodies capable of binding specifically to antigens can be harnessed to manufacture probes for detection
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